Аннотация
В исследовании были проанализированы структурные особенности резидентных макрофагов печени на фоне стойкой артериальной гипертензии, в сравнении с нормотензивным контролем. Для выявления резидентных макрофагов на образцах печени девятимесячных самцов крыс SHR и Wistar (n = 14) применяли иммуногистохимическую реакцию против белка Iba-1. Морфометрические параметры и характер пространственного распределения клеток Купфера оценивали с помощью программ математической обработки и анализа изображений ImageJ и GIMP. Показано, что клетки Купфера в образцах печени крыс линии SHR имеют преимущественно слабоотросчатую либо элипсоидную форму, и не имеют определенной корреляции с расположением в печеночном ацинусе, в отличие от макрофагов группы Wistar. Статистически значимые различия обнаружены в характере распределения клеток Купфера: в группе SHR клетки в печеночном ацинусе распределены более равномерно по сравнению с клетками группы Wistar, наиболее выраженная плотность распределения которых фиксировалась в интермедиальной зоне ацинуса. Обнаруженные структурно-функциональные особенности резидентных макрофагов печени крыс SHR могут быть обусловлены функциональными нарушениями в печени на фоне стойкой артериальной гипертензии.
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