Аннотация
Методами флуоресцентной конфокальной микроскопии изучали влияние специфических блокаторов (-конотоксин GVIA, -агатоксин IVA, нитрендипин, SNX-482, мибефрадил) потенциал-зависимых Са2+-каналов N, P/Q, L, R, T-типов, а также ингибитора фермента гликоген-синтазы киназы-3 GSK3 (1-азакенпауллон) на процессы экзо-эндовезикулярного цикла в холинергических нервно-мышечных синапсах соматической мышцы дождевого червя Lumbricus terrestris. В механизмах везикулярного цикла участвуют ионы Са2+, входящие в терминали через все типы потенциал-зависимых Са2+-каналов пресинаптической мембраны. При этом наибольший вклад в процессы эндоцитоза вносят Са2+-каналы N-, P/Q- и L-типов, тогда как экзоцитоза только каналы N- и P/Q-типов. В процессах рециклинга существенную роль играет динамин-зависимый эндоцитоз, а восстановление везикулярных пулов в таких синапсах, преимущественно, происходит при участии клатрин-зависимого эндоцитоза. Можно считать, что базисные механизмы регуляции везикулярного цикла в двигательных нервно-мышечных синапсах являются общими для всего филогенетического древа позвоночных и беспозвоночных животных, начиная с аннелид. При этом значение отдельных регулирующих элементов машины везикулярной секреции у аннелид имеет свою отчетливую специфику.
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