Аннотация
В настоящее время основным способом отбора наилучших эмбрионов для переноса в рамках вспомогательных репродуктивных технологий (ВРТ) является морфологическая оценка. Для стандартизации процесса отбора с целью увеличения эффективности переноса необходима разработка других объективных и неинвазивных методов. Одним из методов может служить оценка содержания растворимых факторов в образцах сред, полученных при инкубации эмбрионов. Целью исследования явилось определение цитокинов IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IP-10, G-CSF и GM-CSF в кондиционированных эмбрионами средах, а также оценка влияния этих сред на функциональную активность эндотелиальных клеток. Мы не обнаружили стабильного содержания перечисленных цитокинов в кондиционированных эмбрионами средах после 2 суток инкубации. Тем не менее, среды, полученные от эмбрионов качества А и АВ, состоящих из 3 или 4 клеток, оказывали эффект на миграцию эндотелиальных клеток. В дальнейшем необходимо определить природу активных молекул, присутствующих в составе кондиционированных эмбрионами сред, которые могут служить потенциальными маркерами для выбора лучших эмбрионов.
Литература
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