Аннотация
Известно, что нарушения функций астроцитов могут приводить к изменению возбудимости нейронов и, как следствие, к развитию эпилепсии, однако данный вопрос требует дальнейшего изучения. Целью настоящей работы являлась оценка функционального состояния астроцитов гиппокампа у крыс линии Крушинского-Молодкиной (КМ) с наследственной предрасположенностью к рефлекторным аудиогенным судорогам. В экспериментах были использованы «наивные» взрослые животные с полностью сформированной судорожной готовностью. В качестве контроля использовали крыс материнской популяции Вистар. Полученные результаты не выявили различий в экспрессии астроцитарных белков GFAP, ALDH1L1 и NFIA, что свидетельствует об отсутствии в гиппокампе крыс КМ реактивного астроглиоза. Экспрессия SPARC и аквапорина 4 также не отличалась от контрольного уровня, указывая на отсутствие нарушений в астроцитарной регуляции синаптогенеза и водного транспорта. Анализ глиальных белков, отвечающих за обратный захват и метаболизм нейромедиаторов, выявил нормальный уровень экспрессии транспортёра ГАМК GAT-3 и глутаминсинтетазы, однако содержание транспортёров глутамата EAAT1 и 2 было достоверно повышено, что свидетельствует о более активной утилизации синаптического глутамата. Кроме того, сниженная экспрессия гликолитического фермента альдолазы С в гиппокампе крыс КМ, вероятно, указывает на недостаточную активность метаболизма глюкозы. Таким образом, полученные данные выявили генетически детерминированные изменения функционального статуса астроцитов в гиппокампе крыс с наследственной рефлекторной эпилепсией.
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