Аннотация
В условиях эксперимента in vitro исследовали влияние холодового шока на эритроциты морского ерша (Scorpaena porcus L., 1758). Образцы крови содержали при 22оС (контроль) и 4оС (опыт) (градиент 18оС). Экспозиция составляла 3 ч. Холодовой шок не оказывал значимого влияния на показатели жизнедеятельности эритроцитов морского ерша. Клетки сохраняли целостность цитоплазматических мембран, потенциал митохондрий и уровень окислительных процессов, о чем свидетельствовали постоянные значения интенсивности флуоресценции пропидиум йодида (PI), родамина123 (R123) и 2-7-дихлорфлуоресцеин-диацетата (DCF-DA). Основные изменения были связаны с формой эритроцита и функциональной активностью ядра. Клетки приобретали округлые очертания, размеры ядра увеличивались, что привело к росту ядерно-цитоплазматического отношения (NCR) на фоне снижения интенсивности флуоресценции SyberGREENI. Допускается, что это связано с изменением состояния цитоскелета и активизацией транскрипционных процессов.
Литература
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