Аннотация
Предприняты поиск и идентификация осмотически активных белков (ОАБ) в сыворотке крови атлантической трески Gadus morhua с использованием методов электрофореза в полиакриламидном геле и масс-спектрометрии MALDI. Было идентифицировано 17 ОАБ. В соответствии с аннотациями генной онтологии кандидатов, 13 ОАБ отнесены к внеклеточным, а 4 ОАБ – к внутриклеточным белкам. Относительное содержание ОАБ в сыворотке трески составило ~50% от общего белка. В пуле ОАБ доминировали внеклеточные белки аполипопротеины (в составе липопротеинов высокой плотности) и гемопексины, причем относительное содержание первых составило ~25% общего белка сыворотки. Из внутриклеточных белков на протеомной карте сыворотки доминировали низкомолекулярные фрагменты тяжелой цепи миозина. Полученные результаты согласуются с положениями «безальбуминовой» гипотезы капиллярного обмена, которая в качестве осмотически активных белков плазмы «безальбуминовых» костистых рыб рассматривает множественные внеклеточные и внутриклеточные белки разных функциональных классов.
Литература
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