РОЛЬ P2Y-РЕЦЕПТОРОВ В РЕГУЛЯЦИИ АТРОФИЧЕСКИХ ПРОЦЕССОВ В M. SOLEUS ПРИ ФУНКЦИОНАЛЬНОЙ РАЗГРУЗКЕ
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Ключевые слова

m. soleus
атрофия
Е3-лигазы MuRF1 и MAFbx
убиквитин
интенсивность синтеза белкаинтенсивность синтеза белка
P2Y2-рецепторы

Аннотация

Атрофия скелетных мышц при их функциональной разгрузке обусловлена снижением белкового синтеза и резким увеличением его распада. Накопление АТФ в мышце при разгрузке, обнаруженное на ранних сроках, может быть одним из стимулов, запускающих этот процесс. Показано, что паннексиновые каналы пропускают АТФ при разгрузке мышц из цитоплазмы во внеклеточное пространство. Внеклеточный АТФ может восприниматься P2Y2-рецепторами. Для проверки гипотезы об участии P2Y2- рецепторов в регуляции сигнальных процессов в скелетных мышцах на ранних этапах функциональной разгрузки был применен селективный ингибитор P2Y2-рецепторов AR-C 18925XX. Ингибирование P2Y2-рецепторов AR-C 18925XX при 3-дневной функциональной разгрузке крыс снижает атрофию m. soleus, предотвращает накопление АТФ в m. soleus, замедляет экспрессию мРНК Е3-лигазы MAFbx, убиквитина и рецепторов IL6, способствует повышению уровня фосфорилирования AMPK, а также увеличивает интенсивность белкового синтеза.

https://doi.org/10.31857/S0044452922060134
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