Аннотация
Целью работы было изучение распределения нейронов, содержащих GABA, и уровня экспрессии GAT1 в разных слоях неокортекса крыс в неонатальный период после перинатальной гипоксии. Воздействие гипоксии на мозг новорожденных крыс осуществляли на 2-е неонатальные сутки в течение 1 ч при содержании кислорода в дыхательной смеси - 7.8%. Были применены иммуногистохимические реакции на выявление GABA и GAT1. Изучали соматосенсорную область неокортекса на 5 и 10 постнатальные сутки (П5, П10). Показано, что на ранних сроках неонатального периода (П5) воздействие перинатальной гипоксии приводит к увеличению числа тормозных интернейронов во всех слоях неокортекса. К концу неонатального периода (П10) их численность в слоях коры снижается, а в слое V значительно сокращается.
На протяжении неонатального периода у контрольных животных во всех слоях неокортекса уровень экспрессии GAT1 постепенно повышается, после воздействия перинатальной гипоксии происходит значительное снижение экспрессии GAT1, в том числе, в слоях неокортекса, где количество GABAергических нейронов соответствует контрольному значению. Эти факты свидетельствуют о том, что воздействие острой перинатальной гипоксии может приводить к изменению синаптической трансмиссии GABA в всех слоях неокортекса в конце неонатального периода.
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