Аннотация
Методом ОТ-ПЦР в реальном времени проведено исследование сезонных изменений содержания мРНК гигантских белков саркомерного цитоскелета титина и обскурина в скелетной мышце m. longissimus dorsi и левом желудочке сердца длиннохвостого суслика Urocitellus undulatus. Эксперименты проводились на животных следующих экспериментальных групп: «летняя активность», «осенняя активность», «гипотермия» (спячка) и «зимняя активность»; n=5 для каждой группы. В сердечной мышце сусликов группы «гипотермия» обнаружено уменьшение (на 28%, р<0.01) содержания мРНК титина; в трёх других группах статистически значимых различий в этом параметре не выявлено. В m. longissimus dorsi сусликов из групп «гипотермия» и «зимняя активность» наблюдалось увеличение в 2.9 раза (р<0.01) и в 3.6 раза (р<0.01) соответственно содержания мРНК титина при отсутствии статистически значимых различий в этом параметре между группами «летняя активность» и «осенняя активность». Содержание мРНК обскурина было повышено в 3.4-3.6 раз (р<0.01) в сердечной мышце сусликов из групп «осенняя активность», «гипотермия», «зимняя активность» и в 3.0 и 3.6 раза (р<0.01) в скелетной мышце сусликов из групп «гипотермия» и «зимняя активность», соответственно. Таким образом, впервые получены данные о дифференциальной экспрессии мРНК титина и обскурина, указывающие на сопряженные изменения этих белков в мышцах длиннохвостого суслика в период гибернации. Полученные результаты обсуждаются в контексте адаптации поперечнополосатых мышц длиннохвостого суслика к условиям зимней спячки.
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