Abstract
In this work the retinol and a-tocopherol content in bats that living and wintering on the northern periphery of their ranges, at different stages of hibernation and during summer activity was studied for the first time. A characteristic peculiarity of the northern zone bats is staying in hypobiosis for most of the year. The cycles of torpor–arousal during hibernation are associated with a rapid increase in body temperature and respiration, which leads to an increase in the formation of reactive oxygen species. In order to study the antioxidant status in bats of five species, the retinol and a-tocopherol content in the liver and skeletal muscle was determined by HPLC. The study objects were Myotis brandtii, Myotis Mystacinus, Myotis Daubentonii, Plecotus auritus and Eptesicus nilssonii. It was revealed that the antioxidants content was higher in torpid bats during hibernation compared with active animals in summer. At the beginning of hibernation the highest a-tocopherol content in the liver was found in Eptesicus nilssonii, and retinol content in Plecotus auritus. The retinol and a-tocopherol levels in the liver and skeletal muscle of Myotis brandtii in spring was higher than in other species. The a-tocopherol level in the skeletal muscle often exceeded the level detected in the liver at different stages of hibernation. Females showed in the spring, before the end of hibernation, higher antioxidant level in tissues than males. Significant variability of indices was observed in all bat species, which can be explained by both species and individual differences of animals living in natural conditions. High tocopherol and retinol levels in tissues may play an important role in the strategy of antioxidant protection in bats of the northern zone during hibernation.
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