Аннотация
Настоящая работа посвящена изучению механизмов регуляции конститутивного андростанового рецептора (CAR) при воздействии донора оксида азота (NO) S-нитрозоглутатиона (GSNO) in vitro. Исследование выполнено на клетках линии Сасо-2, которые инкубировали в питательной среде в присутствии S-нитрозоглутатиона (диапазон концентраций 1 - 500 мкМ) в течение 3, 24 и 72 ч. Относительное количество CAR оценивали методом вестерн-блот. В работе показано, что кратковременное воздействие донора NO GSNO в течение 3 и 24 ч в концентрациях 1 - 500 мкМ не влияло на количество CAR, а при экспозиции 72 ч с GSNO в концентрациях 1, 10 и 50 мкМ отмечалось его увеличение. Доказано, что повышение относительного количества CAR при воздействии низких концентраций GSNO (1 мкМ) реализуется через NO-цГМФ-сигнальный путь. При увеличении концентрации GSNO до 10 и 50 мкМ отмечается развитие нитрозативного стресса и регуляция CAR осуществляется через битирозин. Прогрессирование нитрозативного стресса (концентрация GSNO 100 и 500 мкМ) приводит к снижению относительного количества CAR, предположительно, вследствие повреждения его молекулы.
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