Аннотация
Дефицит витамина D3 рассматривают в качестве фактора риска при развитии нейродегенеративных заболеваний, сопровождающихся повышенным образованием апоптотических субстратов. В ткани мозга млекопитающих первичным сенсором апоптотических субстратов являются астроглиальные клетки, обладающие фагоцитарной активностью. В настоящей работе был изучен вопрос о влиянии витамина D3 в составе коммерческих препаратов «ДЕТРИМЕД D3» и «Витамин D3 2000МЕ» на фагоцитарную активность астроцитов мозга крысы в первичной культуре. Впервые установлено, что витамин D3 является мощным природным ингибитором фагоцитарной активности астроцитов. Предварительная инкубация астроцитов с 10 мкм витамина D3 приводила к снижению фагоцитарной активности астроцитов (в 1.9 и 3.5 раза по отношению к контролю, при использовании препаратов «ДЕТРИМЕД D3» и «Витамин D3 2000МЕ» соответственно). Этот эффект сопровождался достоверным истощением активного расщепляющего компартмента астроцитов, к которому относятся компоненты эндо-лизосомальной системы (на 31% по сравнению с контролем). Результаты проведенного исследования могут быть использованы в клинической практике для корректировки существующих стратегий лечения c применением витамина D3 у пациентов, страдающих нейродегенеративными заболеваниями, сопровождающимися накоплением апоптотических субстратов.
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