Аннотация
Полное и своевременное удаление апоптотических клеток и их фрагментов является жизненно важным для поддержания гомеостаза во всех тканях организма в норме и при патологических состояниях. Поскольку удаление апоптотических субстратов осуществляется клетками, обладающими фагоцитарной активностью, вопрос о регуляции последней представляет значительный интерес. В настоящей работе исследовали влияние двух биологически активных молекул – инсулина и ганглиозида GM1 на фагоцитарную активность астроцитов мозга крысы в первичной культуре. Показано, что инкубация клеток с 1 мкМ инсулином приводила к достоверному снижению фагоцитарной активности астроцитов (до 58.5% к контролю), а с 10 мкМ ганглиозидом GM1, напротив, вызывала повышение фагоцитарной активности клеток (133.4% к контролю). Предварительная инкубация астроцитов мозга с ганглиозидом GM1 полностью блокировала ингибиторный эффект инсулина. Результаты проведенного исследования могут быть использованы при выработке новых стратегий лечения нейродегенеративных заболеваний, сопровождающихся появлением субстратов апоптотического типа.
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